NCX1 represents an ionic Na+ sensing mechanism in macrophages
Fig 3
MΦs express a novel Slc8a1 splice variant.
(A) A representative real-time amplification plot of Slc8a1, Slc8a2, and Slc8a3 in BMDMs, RAW264.7 MΦs, and brain tissue out of two experiments. (B) Shown are the alignments of RNA-seq reads of BMDMs and their coverage with annotated (gray; GENCODE: ENSMUST00000086538.9, ENSMUST00000163123.1, ENSMUST00000163680.8), predicted (blue; RefSeq PREDICTED: XM_006523944), and StringTie-assembled (red; StringTie Assembly version 1, StringTie Assembly version 2) Slc8a1 splice variants. For numerical raw data, please see S1 Data. The RNA-seq data can be found in the GEO database (https://www.ncbi.nlm.nih.gov) under accession number GSE136662. BMDM, bone marrow–derived MΦ; GEO, Gene Expression Omnibus; MΦ, monocyte/macrophage-like cell; RNA-seq, RNA sequencing; Slc8, solute carrier family 8.