Expression of a recombinant, 4'-Phosphopantetheinylated, active M. tuberculosis fatty acid synthase I in E. coli
Fig 3
Recombinant Mtb FAS I activity.
A. NADPH oxidation assay: blank denotes no enzyme, FAS I denotes FAS I without AcpS, FAS I-AcpS denotes FAS I following AcpS expression, inh denotes FAS I following AcpS expression with 5-ClPZA at 200 μg/ml. B. Ellman’s reaction: blank, FAS I, and FAS I-AcpS as in A. Each plot represent two triplicate batch experiments. Error bars represent standard errors.