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Diversity and Evolutionary History of Iron Metabolism Genes in Diatoms

Fig 3

Diatom ferritin is evolutionarily distinct from eukaryotic ferritin.

Midpoint-rooted maximum likelihood (ML) tree generated with RAxML using PROTGAMMAWAG model. Numbers at major nodes indicate ratio of bootstrap support values from 1,000 trees; values less than 0.5 removed for clarity. At major nodes, where the branching structure is in agreement with Bayesian inference, posterior probabilities are listed to the right of bootstrap values following a forward slash. Consensus trees were generated with MrBayes v3.1.2 from 1,000,000 generations, with trees sampled every 500 generations. Clades colored by dominant organismal phylogeny: diatoms, orange; mixed clades, grey; cyanobacteria, blue-green; haptophytes and cryptophytes, purple; rhodophytes, red; chlorophytes, green; opisthokonts, brown; alveolates, blue; and heterotrophic bacteria, yellow. Ratio of labeled phylogeny over total taxa in group given beside wedge label. Nodes with multiple nodes from the same taxonomic unit were collapsed. MMETSP and accession IDs for select organisms, clockwise from top: Fibrocapsa japonica, MMETSP1339, [CAMERA:0113935616]; Nanofrustulum sp., MMETSP1361, [CAMPEP:0202481506]; Phaeodactylum tricornutum, [JGI:49895]; Ochromonas sp., MMETSP1105, [CAMERA:0173162144]; Chrysoreinhardia sp., MMETSP1166, [CAMERA:0118895568]; Rhizochromulina marina, MMETSP1173, [CAMERA:0118966858]; Mallomonas sp., MMETSP1167, [CAMERA:0182428246]; Eutreptiella gymnastica, MMETSP0811, [CAMERA:0174363882]; Stygamoeba regulata, MMETSP0447, [CAMERA:0177662056]; Stereomyxa ramosa, MMETSP0439, [CAMERA:0174274258]; Mayorella sp., MMETSP0417, [CAMERA:0174229308]; unclassified prasinophyte, MMETSP1310, [CAMERA:0119118176]; Kryptoperidinium foliaceum, MMETSP0120, [CAMERA:0176017054]; unclassified pedinellale, MMETSP0991, [CAMERA:0171785808]; Kryptoperidinium foliaceum, MMETSP0121, [CAMERA:0176108214], [CAMERA:0176054338], [CAMERA:0176293320], and [CAMERA:0176284846]; Glenodinium foliaceum, MMETSP0118, [CAMERA:0167862290].

Fig 3

doi: https://doi.org/10.1371/journal.pone.0129081.g003