Downregulation of the AU-Rich RNA-Binding Protein ZFP36 in Chronic HBV Patients: Implications for Anti-Inflammatory Therapy
Figure 3
Effect of siRNA-mediated silencing of ZFP36 on cytokine production by T cells.
A. Extent of ZFP36 silencing in PBMCs from healthy donors (n = 4). Total PBMCs from healthy donors were transfected by nucleofection with non-specific siRNA (NC siRNA) or ZFP36-specific siRNA (ZFP36 siRNA). qPCR analysis was used to quantify the expression of ZFP36 (p<0.001). B. Cytokine profiles in the supernatant of transfected and stimulated (anti-CD3/CD28) PBMCs. C. Cytokine profiles in the supernatant of transfected and stimulated (CpG ODN2006) PBMCs. The expression levels (pg/ml) of cytokines that displayed significant in control and ZFP36-silenced PBMCs are shown. * p<0.05; ** p<0.01; *** p<0.001. Data were obtained using cells from 4 different donors.