Multiple origins and modularity in the spatiotemporal emergence of cerebellar astrocyte heterogeneity
Fig 3
Quantitative analyses of clone size and stoichiometry of clone composition.
(A,B) Scatterplots of clone size. (A) E12-P30 clones (green) are overall bigger than E14-P30 clones (orange). This same trend is maintained when comparing HomCs and HetCs within each time point (P < 0.001). E12-P30 HomCs contain more cells compared to E14-P30 clones, whereas HetCs’ size changes do not reach statistical significance (P = 0.081). (B) Triple BG+GLA+WMA clones are the biggest clone type and tend to be smaller when generated later (P = 0.317). Double BG+GLA clones are smaller than triple clones and display the same average size in the 2 data sets (P = 0.750). (C,D) Scatterplots show the number of distinct astroglial types in triple and double clones after E12 (C) or E14 (D) IUE. The insets report the clone-wise stoichiometry, rounded to the first decimal, which shows a joint expansion of GLAs and BG compared to WMAs in triple clones, whereas in double clones, BG prevail over GLAs. **, P < 0.01; ***, P < 0.001; P values are calculated with GEE analysis. n = number of clones. The numerical data used in the figure are included in S1 Data. BG, Bergmann glia; E, embryonic day; GEE, generalized estimating equations; GLA, granular layer astrocyte; HetC, heterogeneous clone; HomC, homogeneous clone; IUE, in utero electroporation; WMA, white matter astrocyte.