The SARS-Coronavirus-Host Interactome: Identification of Cyclophilins as Target for Pan-Coronavirus Inhibitors
Figure 2
Localization of SARS-CoV ORFs and interaction network of virus host protein interactions.
Figure 2A shows an overview of the SARS-CoV ORFs used as the basis for the construction of the viral ORFeome [6]. Individual ORFs were PCR amplified by primers specific for the predicted N- and C- terminal ends including sequences of the GATEWAY cassette. Additionally, hydrophobic sequences were deleted from ORFs containing transmembrane regions. Amino acid positions of these fragments (small bars, not drawn to scale) are given behind the respective ORF name and refer to the starting position of each individual ORF. Hypothetical ORF14 [57] was also subcloned. Figure 2B shows highly confident interaction partners of SARS-CoV ORF as identified by ORFeome-wide Y2H screen. Viral proteins are shown in turquoise, and are connected to direct cellular interaction partners shown in orange.