Expression of P. falciparum var Genes Involves Exchange of the Histone Variant H2A.Z at the Promoter
Figure 2
PfH2A.Z is present in the euchromatin compartment.
(A) Colocalization analysis shows PfH2A.Z overlap with the euchromatin marks H3K9ac and H3K4me3 but not with the subtelomeric heterochromatin marks H3K9me3 and HP1. PfH2A.Z, H3K4me3, H3K9ac and H3K9me3 were detected with specific antibodies. (B) Immunoelectronmicroscopy with anti-PfH2A.Z antibodies indicates nuclear compartmentalization of PfH2A.Z. N = nucleus, C = cytoplasm. (C) Immunoprecipitation (IP) of mononucleosomes with anti-PfH2A.Z (H2A.Z) co-precipitates PfH2A.Z and H3K4me3. IP with pre-immune serum (pI) in parallel verified the specificity of the precipitation. Precipitated material was separated by SDS-PAGE and analysed by western blot. 3D7 whole parasite lysate was used as a positive control (Input). Western blots were probed with anti-PfH2A.Z and anti-H3K4me3 antibodies, respectively.