Dexamethasone treatment of murine auditory hair cells and cochlear explants attenuates tumor necrosis factor-α-initiated apoptotic damage
Fig 2
Effects of DEX and TNF-α on intracellular ROS accumulation and expression of apoptosis-related proteins.
Cells were treated with 10 nM DEX for 6 h or 10 ng/ml TNF-α for 24 h. (A) Measurement of ROS generation using DCF fluorescence intensities. Values are expressed as the means ± SE for three independent experiments, expressed as a fold change of untreated control value. * P < 0.05 compared with the untreated control. (B) Representatives immunoblot of apoptosis-related protein expression. Individual band was quantified by densitometry and normalized to β-actin expression. The values in graphs are represented as fold changes relative to the untreated control, expressed as means ± SE of three independent experiments. * P < 0.05 compared with untreated control.