Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish
Fig 2
Solid-Phase Extraction Results.
Separate 5 g fish extracts from a fish containing no measurable ciguatoxin were spiked to a final concentration of either 0.1 (open squares), 0.25 (open diamonds), or 0.5 (open circles) ppb P-CTX-3C. Comparable buffer samples were similarly spiked with of either 0.10 (solid squares), 0.25 (solid diamonds), or 0.50 (solid circles) ppb P-CTX-3C. The control was tissue extract with no ciguatoxin added. Spiked and control samples were serially diluted 1:3 and results plotted as % binding versus equivalents of fish tissue extracted (g mL-1). Results indicated that matrix effects in the fish extracts did not interfere with binding of P-CTX-3C.