Mir-184 Post-Transcriptionally Regulates SOX7 Expression and Promotes Cell Proliferation in Human Hepatocellular Carcinoma
Figure 2
Ectopic miR-184 promotes the proliferation of HCC cells.
A. Real-time PCR analysis of miR-184 expression in Hep3B and Huh7 cells stably overexpressing miR-184. B. Effects of ectopic miR-184 on the proliferation of the indicated HCC cell lines, as analyzed by the MTT assay. C. Representative micrographs (left) and quantifications (right) of crystal violet stained cell colonies formed by the indicated HCC cell lines, 10 days after inoculation. D. Effects of ectopic miR-184 on the tumorigenicity of the indicated HCC cell lines, as determined by anchorage-independent growth ability assay. Colonies larger than 0.1 mm were scored. E. Effects of ectopic miR-184 on the cell cycle progression of the indicated HCC cells, as measured by flow cytometry analysis. Each bar represents the mean ± SD of three independent experiments. *P<0.05.