American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin
Figure 2
A–E: Fragment of skin of a patient with LCL, Caratinga, MG, Brazil.
(A, B) Immunohistochemical labeling of amastigotes of Leishmania with an aliquot of a commercial monoclonal anti-Leishmania antibody and the streptavidin-biotin peroxidase method. (A) Low magnification showing a brown background evidenced by the cytoplasm of the epithelial layer cells (arrowheads). Bar = 32 µm. (B) Higher magnification showing intense non-specific staining visible as dark brown cytoplasmic staining of epithelial (arrowheads) and inflammatory mononuclear cells (macrophages) with intracellular amastigotes of Leishmania in the dermis (arrows) Bar = 16 µm. (C,D) Immunohistochemical labeling of amastigotes of Leishmania using dog hyperimmune serum as the primary antibody with the streptavidin-biotin peroxidase method (C) Low magnification showing light-blue stained background. Bar = 32 µm. (D) Higher magnification showing dark-brown-stained intracellular amastigotes of Leishmania within macrophages in the dermis (arrows) and light-blue-stained background. Bar = 16 µm; (A–D) Immunohistochemistry with the streptavidin peroxidase method counter-stained with Harris’s hematoxylin. (E) Observe immunolabeled amastigotes inside macrophages associated areas of tissue debris (arrow) Epithelium (Ep), Dermis (De).