The Ionic and Hydrophobic Interactions Are Required for the Auto Activation of Cysteine Proteases of Plasmodium falciparum
Figure 2
Expression, purification, refolding and processing of Pro-FP2.
A. Purified and refolded Pro-FP2 was activated/processed in acidic condition. The processing of enzyme was followed by 3 hrs and further evaluated by SDS-PAGE and Western blot analysis. The approximate size of Pro-FP2 and FP2 were mentioned in the figure. B. Purified and refolded Pro-FP2 was processed in the presence of different inhibitors. Leupeptin, E-64 and, prodomain of FP2, were used as a cysteine proteases inhibitors. Whereas, PMSF (phenylmethylsulfonyl fluoride), pepstatin and EDTA were used as serine, aspartic and mettalo proteases, respectively. Finally, proteins were evaluated by SDS-PAGE and western blot analysis.