In Vitro Aggregation Behavior of a Non-Amyloidogenic λ Light Chain Dimer Deriving from U266 Multiple Myeloma Cells
Figure 2
Light chain structural changes induced by pH, denaturant addition and temperature.
(A) CD spectra for the 0.3 g/L light chain solution in 25 mM PBS at pH 7.4 (―) and in 20 mM HCl at pH 2.0 (–); (B) Fraction of unfolded protein as a function of guanidinium hydrochloride (GuHCl) concentration evaluated by intrinsic tryptophan fluorescence measurements (see Materials and Methods). The continuous line represents the interpolation of experimental data according to Eq. 1; (C) Fraction of unfolded protein as a function of temperature evaluated by CD measurements (see Materials and Methods). The continuous line corresponds to the interpolation of experimental data according to Eq.1.