Generation of a Novel Bacteriophage Library Displaying scFv Antibody Fragments from the Natural Buffalo Host to Identify Antigens from Adult Schistosoma japonicum for Diagnostic Development
Fig 2
Binding of buffalo portal-round three selected-adult and ES pooled (Bp-R3-AES) single-chain Fv domain (scFv) phage library to Schistosoma japonicum protein extracts.
Data represent mean ± S.E.M. of the O.D.450nm for Bp-R3-AES phage binding to soluble whole adult preparations (SWAP) (A) and soluble egg antigens (SEA) (B). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test, where * P < 0.05. Antigens from SWAP (Lane 1), and SEA (Lane 2) of S. japonicum were probed with Bp-R3-AES phage (C). Molecular weights in kilodaltons (kDa) are indicated on the left hand side. Regions of intense binding by Bp-R3 scFv phages to antigen preparations is indicated by arrows.