Extensive Regulation of Diurnal Transcription and Metabolism by Glucocorticoids
Fig 2
Constant DEX treatment rescues diurnal gene expression patterns especially of metabolic genes.
(A) Sampling and treatment schedules of the transcriptomics and metabolomics studies. Zebrafish embryos/larvae were raised under 12 h light (yellow) / 12 h dark (black) cycles. dpf: days post fertilization. ZT: Zeitgeber Time in hours after lights on (ZT0); lights off: ZT12. DEX: dexamethasone. (B) Model assignments under DEX rescue. Black curved line: oscillation with same phase and amplitude as wild-type, red curved line: oscillation with a phase or amplitude different from wild-type. (C) Genes defined as rescued. Rescued genes of all “affected” models in the untreated situation (5,6,14,15) that are now classified as model D under DEX treatment (= showing identical phase and amplitude in both phenotypes) are indicated as dark blue. Genes classified as model E under DEX treatment that were either not rhythmic in the untreated mutant situation (model 5 and 6) or that were rhythmic (models 14 and 15), but showed higher phase or amplitude differences in the untreated than in the treated situation, are indicated as light blue. Genes of model 14 and 15 now classified as model E, but with no reduction of phase or amplitude differences are named model E* and indicated by dark grey. Model E* genes are not considered as rescued. (D) Pie charts depicting the behaviour of model 5-6-14-15 genes under DEX treatment. Genes not rescued by DEX treatment are indicated by the dark (model E*) and light (models other than D or E) grey colours. (E) Enriched metabolic pathways among the rescued genes.