Multiple origins and modularity in the spatiotemporal emergence of cerebellar astrocyte heterogeneity
Fig 2
Composition of clones derived from E12 or E14 progenitors.
(A-C) HomCs composed of WMAs (A; arrowheads indicate sister cells), GLAs (B), or BG (C) are generated by both early- and late-tagged progenitors. (D,E) The 2 major types of HetCs include triple clones made of BG+GLA+WMA (D) and double clones made of BG+GLA (E). (F,G) HomCs (dark shade) and HetCs (light shade) are produced in different frequencies by E12 (F) and E14 progenitors (G, P = 0.009). Among HetCs, BG+GLA+WMA clones are more numerous in E12-P30 compared to E14-P30 clones (P = 0.023), whereas BG+GLA clones do not vary (P = 0.748). Whereas WMA HomCs double in frequency in E14-P30 clones compared to E12-P30 clones (P < 0.001), BG HomCs decrease significantly (P = 0.021). Pies illustrate pooled data from 3–4 animals. (F) Minor fractions: CNA = 3.52%, WMA+CNA = 2.47%, GLA+CNA = 0.35%, BG+WMA = 1.06%, WMA+GLA = 3.17%, BG+GLA+ WMA+CNA = 2.47%. (G) Minor fractions: CNA = 3.92%, WMA+CNA = 0.98%, BG+WMA = 1.96%, WMA+GLA = 2.94%. P values are calculated with Fisher’s exact test. n = number of clones. Green = E12-P30 clones; orange = E14-P30 clones. Scale bars: 30 μm. BG, Bergmann glia; CNA, cerebellar nuclei astrocyte; E, embryonic day; GL, granular layer; GLA, granular layer astrocyte; HetC, heterogeneous clone; HomC, homogeneous clone; P, postnatal day; PCL, Purkinje cell layer; WM, white matter; WMA, white matter astrocyte.