Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Fig 2
Effects of GRT on proliferation of enzalutamide-resistant C4-2 MDV3100r, PC-3, and non-cancerous human cell lines.
Proliferation of LNCaP C4-2 MDV3100r (A) and PC-3 (B) being treated with increasing concentration of GRT (0–100 μg/ml for C4-2 MDV3100r cells and 0–200 μg/ml for PC-3 cells) for 96 h was determined by Hoechst 33258 proliferation assay. (C) Morphology of C4-2 MDV3100r cells under treatment of different concentrations of GRT (0, 50, 75, 100 μg/ml) was examined by microscope. The magnification of the microscope is 100X and the yellow scale bar represented 100 μm. (D) Proliferation of HUVEC and HEK293 cells being treated with increasing concentration of GRT (0–100 μg/ml) was determined by Hoechst 33258 proliferation assay. Asterisk *, ** and *** represents statistically significant difference p < 0.05, p < 0.01 and p < 0.001, respectively, between the two groups of cells being compared.