Operator Dependent Choice of Prostate Cancer Biopsy Has Limited Impact on a Gene Signature Analysis for the Highly Expressed Genes IGFBP3 and F3 in Prostate Cancer Epithelial Cells
Figure 2
Scatterplots of gene expression levels derived from the primary and the secondary cancer sample measurements.
In order to measure how similar the primary and secondary cancer samples were in terms of gene expression levels, and further for the outlier analysis, we generated scatterplots of gene expression levels of the two measurements. The expression levels of the three signature genes are shown as delta Ct values using the same equation as described in the legend of Figure 1. The primary and secondary cancer sample measurements refer to the two measurements containing the first and second most common Gleason patterns. The two types of cancer samples from 43 patients are compared using scatterplots. Red circles indicate the outliers, which were further investigated regarding tissue input quantity and percentages of cancer epithelial cells. Dashed line square indicates VGLL3 measurements that had high delta Ct values and poor reproducibility.