Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis
Figure 2
Schematic diagram showing the construction strategy of pD5Dselect.
The pRbcS450 plasmid (A) contains the selection cassette, flanked by NotI restriction sites. The pJET-LiD5Des plasmid (B) contains the functional DES5 gene with its flanking regions. Following NotI digestion, the selection cassette was subcloned into pJET-LiD5Des. Primers used in analysis of P127-transformant are marked.