Broad-Spectrum Transgenic Resistance against Distinct Tospovirus Species at the Genus Level
Figure 2
Comparison of tospoviral L gene region encoding RNA-dependent RNA polymerase (RdRp) conserved motifs of L protein.
(A) Alignment of L gene sequences corresponding to the RdRp motifs, A, B, C, D and E of L proteins of Watermelon silver mottle virus (WSMoV), Capsicum chlorosis virus (CaCV), Groundnut bud necrosis virus (GBNV), Melon yellow spot virus (MYSV), Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus (INSV). (B) The recognizability of the L RNAs of WSMoV, TSWV and INSV by the 32P-labeled probe prepared using the cloned L gene conserved region (WLm), as analyzed by northern hybridization. Genomic RNAs of Zucchini yellow mosaic virus (ZYMV), Turnip mosaic virus (TuMV), Papaya ringspot virus (PRSV) and Cucumber mosaic virus (CMV) were also used in the hybridization analysis. RNA from mock inoculated plant was used as the negative control. Ribosomal RNAs indicate the loading controls.