Amoebal Endosymbiont Neochlamydia Genome Sequence Illuminates the Bacterial Role in the Defense of the Host Amoebae against Legionella pneumophila
Figure 2
Amoebae harboring Neochlamydia S13 are completely protected from L. pneumophila infection.
(A) L. pneumophila survival in Neochlamydia S13-infected amoebae (S13WT) and aposymbiotic amoebae (S13RFP). (B) L. pneumophila growth in Protochlamydia R18-infected amoebae (R18WT) and aposymbiotic amoebae (R18DOX). (C) L. pneumophila survival in C3 reference strain amoebae (C3), and PYG medium only (Med). Data are the means ± SD from at least three independent experiments performed in triplicate. * denotes p<0.05 at each of the time points. (D) Representative Gimenez staining images showing L. pneumophila replication into amoebae at 5 days post-infection. Arrows, Neochlamydia S13 (S13WT) or Protochlamydia R18 (R18WT). Arrowheads, replicated L. pneumophila.