The Attenuated Brucella abortus Strain 19 Invades, Persists in, and Activates Human Dendritic Cells, and Induces the Secretion of IL-12p70 but Not IL-23
Figure 2
Human immature DCs are activated by B. abortus S19.
Monocyte-derived immature DCs were infected with B. abortus S19 (MOI, 20) for 1 h, the bacteria were washed out, and the cells were incubated for another 48 h. Control cells were left uninfected. (A) After 48 h, the phenotype of the cells was determined by flow cytometry (bold lines, infected cells; grey areas, uninfected cells; dotted lines, isotype controls). One representative of at least six independent experiments is shown. (B) Supernatants were analyzed for the presence of IL-12/23p40, IL-12p70, IL-10, and TNF-α. Data expressed as median with interquartile range.