Whole Methylome Analysis by Ultra-Deep Sequencing Using Two-Base Encoding
Figure 2
Comparison of the sequencing coverage for bis-sol, bis gel and a non-bisulfite converted DH10B sequencing run.
Plots A (bis-sol) and B (bis gel) show the coverage of sequencing reads against the +/− bisulfite converted references (dark/light blue) and the normal reference (red). As a comparison, plot C shows the coverage of a regular non-bisulfited DH10B sequencing run matched towards the normal reference (yellow) (Data from SOLiD software communtiy web-site). The missing coverage in all three plots corresponds to a 133 kb perfect repeat in tandem. Since reads will match to both repeats, they will not show up under the unique matches.