Testing of library preparation methods for transcriptome sequencing of real life glioblastoma and brain tissue specimens: A comparative study with special focus on long non-coding RNAs
Table 6
Minimum requirements and brief characterization of three tested library preparation kits.
The minimum requirements according to manufacturers' protocols are the quality of pre-depletion RNA and the amount of input rRNA-depleted RNA. The quality of RNA is based either on RNA integrity number (RIN) or the source of RNA, for example formalin-fixed paraffin-embedded (FFPE) tissue blocks. Preceding depletion of rRNA is required for all library prepration procedures.