Testing of library preparation methods for transcriptome sequencing of real life glioblastoma and brain tissue specimens: A comparative study with special focus on long non-coding RNAs
Table 5
Summary scores of chosen parameters of all three tested library preparation kits.
Scores were assigned in four categories, specifically the percentage of rRNA reads and duplicate reads and the number of hits in Ensembl database for the groups of mRNAs and lncRNAs. The lowest score in each category is denoted by “-” and the highest by “++”. Based on these parameters, the most suitable kit for preparation of libraries for sequencing of lncRNAs, NEBNext, would be chosen and later independently tested in another NGS facility.