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Agrobacterium tumefaciens-mediated transformation of a hevein-like gene into asparagus leads to stem wilt resistance

Fig 13

Process of producing transgenic Asparagus plants.

1: Induction of embryogenic callus; 2: Induction of embryos; 3: A. tumefaciens-mediated genetic transformation of asparagus embryos; 4: Screening of A. tumefaciens-mediated genetic transformation of embryos; 5: Induction of screened transformants; 6: Production of transgenic seedlings; 7: Outdoor transplantation.

Fig 13

doi: https://doi.org/10.1371/journal.pone.0223331.g013