Melt Analysis of Mismatch Amplification Mutation Assays (Melt-MAMA): A Functional Study of a Cost-Effective SNP Genotyping Assay in Bacterial Models
Figure 12
Allele-Specific MAMAs Based Upon Agarose Sizing.
Allele-specific SNP MAMAs from two pathogen species were transferred from a real-time PCR instrument (A & C; Melt-MAMA) to conventional PCR coupled with agarose gel electrophoresis (B & D; Agarose-MAMA) under identical assay conditions. Genotypes from Melt-MAMAs are determined by temperature-dissociation (melt) curves, whereas genotypes from Agarose-MAMAs are determined by the amplicon size differences.