Light Directs Zebrafish period2 Expression via Conserved D and E Boxes
Figure 12
The LRM mediates TEF and CLOCK:BMAL activation in vitro.
COS-7 cells were transfected with wild-type and mutated −0.43per2:luciferase constructs. Mutations include the LRM-E-box (−0.43per2-ME:luc) and the LRM-D-box (−0.43per2-MD:luc). The cells were co-transfected with combinations of expression vectors (indicated by +) of TEF and BMAL2/CLOCK1. Transcriptional activity is expressed as relative luciferase activity (mean±SE). Results are the mean of three independent experiments performed in triplicate. Statistical analysis was performed by three-way ANOVA. These results imply that both the BMAL/CLOCK complex and TEF contribute to the LRM activation.