Involvement of a Velvet Protein FgVeA in the Regulation of Asexual Development, Lipid and Secondary Metabolisms and Virulence in Fusarium graminearum
Figure 12
Yeast two-hybrid analysis of the interaction between FgVeA, FgVelB and FgLaeA1 or six FgVeA interacting proteins (named FgVIP1-6).
The pair of plasmids pGBKT7-53 and pGADT7 was served as a positive control. The pairs of plasmids pGBKT7-Lam and pGADT7, pGADT7 and pGBKT7-FgVeA were used as negative controls. Growth of the transformed yeast was assayed on the medium containing 5 mM 3-aminotriazole (3-AT), but lacking His, Leu and Trp. Columns in each panel represent serial decimal dilution.