Alzheimer’s risk factor FERMT2 promotes the progression of colorectal carcinoma via Wnt/β-catenin signaling pathway and contributes to the negative correlation between Alzheimer and cancer
Fig 10
Aβ1–42 treated HT22 cells inhibited the migration and invasion of CT26 cells through the Wnt/β-catenin signaling pathway.
(A, B) CT26 cells were transfected with β-catenin overexpression plasmid. The protein levels of β-catenin and β-actin were detected by Western blot analysis. (C, D) The HT22 cells were treated with or without Aβ1–42 (40 μM) and co-cultured with CT26 cells transfected with β-catenin overexpression or control plasmid. The migration and invasion abilities of CT26 cells were determined by the transwell assays. Scale bars: 100 μm. (E) The CT26 cells were transfected with β-catenin overexpression or control plasmid and co-cultured with or without the AD model cells. The levels of β-catenin and cyclin D1 proteins in CT26 cells were measured by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001.