Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell–Cell Contact
Figure 10
Env localizes to sites where target cell membranes are anchored in infected cells.
(A-F) HEK293 cells expressing MLV GagPol, genome, MLV Env-YFP (red), and dynamin2-CFP (green) were cocultured with XC target cells expressing mCAT1-CFP (green). MLV Env-YFP efficiently localized to sites of cell–cell contact in addition to being incorporated into punctate virions. Size bars correspond to 15 µm.