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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 610-613Effect of Cell Cycle Phase on Sf9 Cell Activity...

Effect of Cell Cycle Phase on Sf9 Cell Activity and Autographa Californica Multiple Nucleopolyhedrovirus Infection

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Abstract:

The distribution and activity of cell receptors, which are key factors of baculovirus-insect cell interactions, may be attributed to cell cycle. In fact, the virtual difference in time of infection is the difference in cell cycle distribution. In this work, the effects of cell cycle on cell activity and baculovirus production were investigated. Sf9 cells were infected with baculovirus at the different cycle phases. It was found that G1 phase plays a substantial role in cell activity and competence for the baculovirus replication. Sf9 cells have the highest succinate dehydrogenase activity and are most sensitive for the baculovirus replication when the proportion of G1 phase in cell population reaches a maximum. On the hand, cell activity is at the lowest when G2/M percentage reaches its maximum. These results provide a guidance in developing the baculovirus infection dynamics model and controlling the expression of useful foreign genes when cell cycle is taken into account.

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Progress in Environmental Science and Engineering

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Periodical:

Advanced Materials Research (Volumes 610-613)

Pages:

115-119

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.610-613.115

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Online since:

December 2012

Authors:

You Hong Zhang, Wei Wei, Peng Xu, Qin Qin, Jie Chen, Xing Zhou Chen, Xiao Ping Chen, Xi Hong Zhao

Keywords:

Activity, Baculovirus, Cell Cycle, Flow Cytometry, Infection

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