Streptococcus pneumoniae Cell-Wall-Localized Phosphoenolpyruvate Protein Phosphotransferase Can Function as an Adhesin: Identification of Its Host Target Molecules and Evaluation of Its Potential as a Vaccine
Fig 1
A) Immunoblot of untagged rPtsA: total S. pneumoniae extract (Total), total cell-wall proteins (CW), cytoplasmic proteins (Cyto) and membrane extract (mem) were probed with rabbit anti-rPtsA antiserum. B) Immunoblot of recombinant pneumolysin (rPly): total S. pneumoniae extract, total cell-wall proteins, cytoplasmic proteins and membrane extract were probed with rabbit anti-rPly antiserum. C) Immunoblot of tagged rFabD: total S. pneumoniae extract, total cell-wall proteins, cytoplasmic proteins and membrane extract were probed with rabbit anti-rFabD antiserum. D) FACS analysis of PtsA surface expression using anti-rPtsA monoclonal antibody (mAb) and isotype control IgG. Red line–preimmune control serum; green line–anti rPtsA mAb diluted 1:50; blue line–anti rPtsA mAb diluted 1:20. M = molecular weight markers. E) SIM orthogonal view image demonstrating the surface staining with mouse anti rPtsA antibody detected with donkey anti-mouse IgG (green). Three planes XZ, XY and YZ are shown. PtsA is visible on the perimeter of the bacterium in green, surrounding the white bacterial genome.