The Expression and Characterization of Functionally Active Soluble CD83 by Pichia pastoris Using High-Density Fermentation
Figure 1
A schematic genetic map of the sCD83 expression vector.
The sCD83 gene was inserted between the Xho I and EcoR I sites in pPIC9K and in-frame with the Kex2 cleavage site in the sequence of the α-factor secretion signal to create the expression vector pPIC9K-sCD83.