Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences
Figure 1
In silico evaluation of 16S rRNA PCR primers.
A) Percentage of sequences matching individual primers, with the top two primers highlighted in boxes. B) Percentage of sequences amplifiable by various primer pairs (338F*/1061R is the best pair). Percentage of matched sequences is measured against the Greengenes 16S rRNA sequence database. See Table S4 in File S1 for primer sequences and results measured against the RDP and SILVA databases. Primer numbering is based on the E. coli system of nomenclature as in Brosius et al. [37] and for simplicity the same name (say 784F) is used for both forward and reverse primers at a given position.