Naïve Rat NK Cells Control the Onset of T Cell Response
Figure 1
In congenic LEW.BH-NKC the monoclonal antibody 3.2.3 binds to NKR-P1A but not NKR-P1B molecules and allows clear identification of NK cells. A:
Staining of Lewis wt and congenic LEW.BH-NKC bone marrow, blood derived lymphocytes and splenocytes with mAb 3.2.3 led to different recognition pattern in flow cytometry. While cells from Lewis wt showed two peaks indicating the detection two cell populations 3.2.3high and 3.2.3low, congenic LEW.BH-NKC derived cells revealed a comparable percentage of 3.2.3high cells but an extenuated peak of 3.2.3low cells. B: Double staining of mAb 3.2.3 with anti-CD3 and anti-CD172a, a marker for monocytes, respectively. While the 3.2.3high expressing cells remain single positive the cells expressing 3.2.3low were either double positive for CD3 thus can be designated as NKT cells or expressed on CD172a+ monocytes. The 3.2.3+/CD3+ could be also detected in LEW.BH-NKC rats whereas the CD172a+ double positive cells were missing. These data indicate that the 3.2.3high cells are NKR-P1A+ NK cells and the 3.2.3low cells comprise NKR-P1B expressing monocytes or NKT cells. Results are shown as representative data of a typical screen of Lewis wt and LEW.BH-NKC rats.