Environmental Exposure of the Mouse Germ Line: DNA Adducts in Spermatozoa and Formation of De Novo Mutations during Spermatogenesis
Figure 1
B[a]PDE-N2-dG adducts in caput spermatozoa from untreated and exposed male germ cells at different stages of spermatogenesis.
The male germ cell stage is indicated along with day of sacrifice. Untreated cauda spermatozoa = CASZ (number of animals n = 4); epididymal spermatozoa = ESZ (n = 4); spermatids = ST (n = 4); spermatocytes = SC (n = 6); differentiating spermatogonia = Diff. SG (n = 4); stem cell spermatogonia = Stem cell SG (n = 5). B[a]PDE-N2-dG adduct levels are given as means±SE. For untreated CASZ and exposed Diff. SG and Stem cell SG the values were below detection. Statistically significant differences and p-values are indicated in the figure (ANOVA and post hoc LSD).