Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats
Fig 1
Immunoblot of TMPRSS6, HFE2 and ATP1A in the 3000 g fraction.
Detection of TMPRSS6, HFE2 and ATP1A is enhanced in the 3000g fraction of mouse liver homogenates (3000g) in comparison with whole liver homogenates prepared in 1% NP40 (NP40) or microsomes (Micros). Samples were prepared from C57BL/6 mice (C), mask mice (m), Hfe2+/+ mice (WT) and Hfe2-/- mice (KO). Arrows denote the TMPRSS6-, HFE2- and ATP1A-specific bands. In contrast to the NP40 whole liver homogenate or liver microsomes, HFE2 is present in the 3000 g fraction solely as a full-length protein.