Interleukin-13 Inhibits Lipopolysaccharide-Induced BPIFA1 Expression in Nasal Epithelial Cells
Fig 1
LPS induces BPIFA1 expression in nasal epithelial cells.
(A) RPMI-2650 cells were treated with various concentrations (0–20 μg/ml) of LPS for 2 h, and mRNA levels of BPIFA1 were analyzed by quantitative real-time PCR. (B) Cell lysates were then prepared to measure BPIFA1 protein expression levels by western blot after incubation with LPS for 24 h. Protein expression levels of BPIFA1 were quantified by densitometric analysis and normalized to those of β-actin. ANOVA with Tukey’s test was used to compare the overall difference between the groups. *, P < 0.05 compared to LPS-untreated control group.