RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling
Fig 1
(A) Schematic of FLASR (ZZ-rsEGFP2tandem) bound to an immunoglobulin protein. (B-D) Maximum intensity projections of confocal z-stacks of methanol fixed mammalian CV-1 cells immunolabelled with antibodies against β-actin (B), vimentin (C) and α-tubulin (D). Subsequently, purified FLASR (red) was used to decorate the primary antibodies. Nuclei were stained with DAPI (blue). Scale bars: 50 μm.