Unique Haploinsufficient Role of the MicroRNA-Processing Molecule Dicer1 in a Murine Colitis-Associated Tumorigenesis Model
Figure 1
Intestinal-epithelial-cell-specific Dicer gene ablation.
a, Dicer protein expression levels in the isolated primary colonic epithelial cells of Dicerfl/fl control, VillinCre-DicerΔ/+, and VillinCre-DicerΔ/Δ mice. Representative western blotting images of three independent experiments are shown. b, Representative immunohistochemical images of DICER protein expression (in brown) in the colons of control and mutant mice. The sections are 30 mm proximal to the anal canal. Scale bars = 200 µm. Similar results were obtained from at least three independent mice per group. c, d, The expression levels of the indicated miRNAs in isolated colonic epithelial cells of control and mutant mice determined by Northern blotting (c) and qRT-PCR (d). Representative images of three independent experiments are shown (c). U6 was used as the loading control. The data were obtained from three independent experiments and are shown as means ± s.d. after adjusting the value of control mice as 1. *, p<0.05 (d).