APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
Figure 1
APRIL expression is upregulated in CRC tissues and human CRC cell lines.
(A) Western blot analysis of APRIL in eight pairs of colorectal tumors (T) and adjacent normal tissues (N). #1–8 indicates patients' numbers. β-actin was used as an internal control. (B) Semiquantitative RT-PCR analysis of APRIL expression in four human CRC cell lines (SW480, HT-29, Colo-205 and HCT-116). HUVECs were used as a negative control and GAPDH was an internal control. (C) SW480 cells were transfected with shRNA directed against nontargeting control (shNTC) or human APRIL gene (sh637, sh1750), and after 48 h, APRIL mRNA level was determined by semiquantitative RT-PCR. GAPDH was an internal control. (D) SW480 cells were transfected with shNTC or shAPRIL, and after 48 h, APRIL protein levels were determined by western blot. β-actin was an internal control. (E) CRC cell lines and shAPRIL-transfected SW480 cells were cultured for 72 h, and supernatants were collected and assessed for secreted APRIL by ELISA.