Zika virus-like particle vaccine protects AG129 mice and rhesus macaques against Zika virus
Fig 1
Design, production, purification, and characterization of ZIKV VLP vaccine candidate.
(1A) Schematic of ZIKV genome and plasmid DNA encoding ZIKV structural genes: 1) prM derived from the MR766 African strain (orange) with native signal sequence replaced with the human IL2 sequence (yellow) (MYRMQLLSCIALSLALVTNS); 2) E ectodomain derived from the SPH2015 Brazilian strain (grey), and 3) E stem-anchor (S-A) from MR766 (orange). (1B) SDS PAGE Coomassie gel, lane 1, 1μg purified VLP, and lane 2, 2μg purified VLP, approximately 55kDa. (1C) Western blot, lane 1, 0.25μg purified VLP, and lane 2, 0.5μg purified VLP, approximately 55kDa.