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Isolation of aCandida utilis variant by using a nitrogen-limited continuous culture

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Abstract

During continuous culture ofCandida utilis the appearance of a morphologic variant yeast was detected. The new microorganism developed systematically whenever it was changed from normal to stressed propagation conditions. A simple system was used for the isolation of the yeast variant, which was defective in cellular division and showed improved kinetic parameters and oxygen uptake rate. An asynchronic nitrogen-limited continuous culture ofCandida utilis allowed us to enrich the population in the chemostat with the modified yeast and isolate it in a defined medium. Assimilation and fermentation tests indicated it to be a variant ofCandida utilis that showed stable morphologic and physiologic differences with the parental yeast.Candida utilis growing in this nitrogen-limited continuous culture also showed a high mutation rate.

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  1. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), Caseros, Tucumán, Argentina

    Juan C. Diaz Ricci & Francisco Tell

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  1. Juan C. Diaz Ricci
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  2. Francisco Tell
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Diaz Ricci, J.C., Tell, F. Isolation of aCandida utilis variant by using a nitrogen-limited continuous culture. Current Microbiology 17, 43–48 (1988). https://doi.org/10.1007/BF01568818

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  • DOI: https://doi.org/10.1007/BF01568818

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