Summary
Total cellular DNA extracted from eight red algal species (from the genera Gracilaria, Gracilariopsis, Porphyra and Gymnogongrus) was centrifuged on Hoechst dye/CsCl gradients. In five species, plasmid-like DNAs banded with the A+T rich organellar DNAs in the CsCl gradients. Based on their electrophoretic migration in different agarose gels, the plasmid-like DNAs are circular. This is the first report of putative plasmid DNAs in the red algae outside the genus Gracilaria. Two similar Gracilaria chilensis plasmid-like DNAs of 3.8 and 3.4 kb (GC2 and GC3) were cloned in pUC19. The cloned GC2 DNA did not hybridize to either the organellar or nuclear genomes of G. chilensis, suggesting that GC2 is a true plasmid. GC2 did hybridize to the plasmid of one other red algal species, Gracilaria sordida.
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References
Aldrich J, Cattolico RA (1981) Plant Physiol 68:641–647
Armstrong KA, Som T, Volkert FC, Broach JR (1988) In: Kelly T, Stillman B (eds) Cancer cells 6/ Eukaryotic DNA replication. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 213–223
Bailey-Serres J, Leroy P, Jones SS, Wahleithner JA, Wolstenholme DR (1987) Curr Genet 12:49–53
Coggins LW (1987) Preparation of nucleic acids for electron microscopy. In: Sommerville J, Scheer U (eds) Electron microscopy in molecular biology, a practical approach. IRL Press, Oxford, Washington D.C.
Collins RA, Stohl LL, Cole MD, Lambowitz AM (1981) Cell 24:443–452
Crouzillat D, Gentzbittel L, de la Canal L, Vaury C, Perrault A, Nicolas P, Ledoigt G (1989) Curr Genet 15:283–289
Davis LG, Dibner MD, Batley JF (1986) Basic methods in molecular biology, Elsevier, New York
Erickson L, Beversdorf WD, Pauls KP (1985) Curr Genet 9:679–682
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Geier GE, Modrich P (1979) J Biol Chem 254:1408–1413
Goff LJ, Coleman AW (1988a) J Phycol 24:357–368
Goff LJ, Coleman AW (1988b) J Phycol 24 supl. June 88, p 23
Guerineau M, Grandchamp C, Paoletti C, Slonimski P (1971) Biochem Biophys Res Commun 42:550–557
Gunge N, Tamaru A, Ozawa F, Sakaguchi K (1981) J Bacteriol 145:382–390
Johnson PH, Grossman LI (1977) Biochemistry 16:4217–4225
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Manuelidis L (1977) Anal Biochem 78:561–568
Marinus MG, Morris NR (1973) J Bacteriol 114:1143–1150
Metz BA, Ward TE, Welker DL, Williams KL (1983) EMBO J 2:515–519
Pring DR, Lonsdale DM (1985) Intern Rev Cytol 97:1–46
Shikanai T, Yamada Y (1988) Curr Genet 13:441–443
Smith AG, Chourey PS, Pring DR (1987) Plant Mol Biol 10:83–90
Southern EM (1975) J Mol Biol 98:503–517
Turpen T, Garder SJ, Grill LK (1988) Plant Mol Biol 10:489–497
Wahleithner JA, Wolstenholme DR (1987) Curr Genet 12:55–67
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Communicated by R. W. Lee
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Villemur, R. Circular plasmid DNAs from the red alga Gracilaria chilensis . Curr Genet 18, 251–257 (1990). https://doi.org/10.1007/BF00318389
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DOI: https://doi.org/10.1007/BF00318389