Summary
One fertile and two male-sterile diploid plants were regenerated from the same callus after two cycles of protoplast culture from fertile Nicotiana sylvestris. Genetic studies indicated that the male-sterile trait was under cytoplasmic control. Progenies of each regenerated plant possessed different mitochondrial (mt) DNA restriction patterns. Both cms protoclone types were characterized by specific mtDNA deletions. In addition, a 40 kD mitochondrially encoded polypeptide is lacking in the cms plants.
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Li, X.Q., Chetrit, P., Mathieu, C. et al. Regeneration of cytoplasmic male sterile protoclones of Nicotiana sylvestris with mitochondrial variations. Curr Genet 13, 261–266 (1988). https://doi.org/10.1007/BF00387773
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DOI: https://doi.org/10.1007/BF00387773