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Analysis of effector T cells against the murine syngeneic tumor MethA in mice orally administered antitumor polysaccharide SPR-901

  • Original Articles
  • Polysaccharide SPR-901, Antitumor Effect, Oral Administration, T Cell Effector
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Abstract

The growth of MethA tumor was significantly inhibited by oral administration of the α-glucan SPR-901 in BALB/c (+/+) mice but not in nude mice. Mice treated orally with SPR-901 exhibited an augmentation of antigen-specific resistance against rechallenge with the tumor cells. The tumor-neutralizing activity of regional lymph node cells from MethA-bearing mice against the tumor was augmented by oral administration of SPR-901. The tumor-neutralizing activity of lymph node cells from SPR-901-treated mice mainly appeared in Lyt2+cells. Furthermore, lymphokine-activated killer activity of these cells was enhanced by administration of SPR-901. The antitumor effect of SPR-901 was abrogated in mice depleted of either L3T4+ or Lyt2+ cells, and in cyclosporin-A-treated mice. These results suggest that Lyt2+ cells are important effector cells in MethA-bearing mice orally adminstered SPR-901 and that functional exertion of both Lyt2+ and L3T4+T cells is necessary for the antitumor effect of orally administered SPR-901 in vivo.

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Authors and Affiliations

  1. Pharmaceutical Research Laboratories, Sapporo Breweries Ltd., 10 Okatohme, Yaizu, 425, Shizuoka, Japan

    Yasuyuki Takeda, Makoto Tanaka, Hiroyuki Miyazaki & Suguru Takeo

  2. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan

    Kikuo Nomoto

  3. Laboratory of Germ Free Life, Research Institute for Disease Mechanism and Control, School of Medicine, Nagoya University, 466, Nagoya, Japan

    Yasunobu Yoshikai

Authors
  1. Yasuyuki Takeda
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  2. Makoto Tanaka
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  3. Hiroyuki Miyazaki
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  4. Suguru Takeo
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  5. Kikuo Nomoto
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  6. Yasunobu Yoshikai
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Takeda, Y., Tanaka, M., Miyazaki, H. et al. Analysis of effector T cells against the murine syngeneic tumor MethA in mice orally administered antitumor polysaccharide SPR-901. Cancer Immunol Immunother 38, 143–148 (1994). https://doi.org/10.1007/BF01525634

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  • DOI: https://doi.org/10.1007/BF01525634

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