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Granulocyte response to oxidized FMLP

Evidence for partial inactivation of FMLP

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Abstract

We prepared FMoxLP by oxidation of FMLP and evaluated its ability to trigger a variety of granulocyte responses. FMoxLP was found to depolarize granulocyte membranes; increase granulocyte oxygen consumption, superoxide production, and hydrogen peroxide production; compete with FMLP for binding to granulocytes; and stimulate granulocyte chemotaxis. Against all of these granulocyte functions, FMoxLP was considerably less potent than the parent FMLP. When luminol-dependent-granulocyte chemiluminescence (CL) was studied, FMoxLP was observed to be a more potent stimulus of this response than FMLP. This increased CL activity of FMoxLP may be related to nonoxidative burst mechanisms. Our results indicate that FMoxLP retains a significant amount of the biological activity of FMLP (albeit in general less potent). The biological importance of the observed activities of FMoxLP must await further investigation.

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Authors and Affiliations

  1. Departments of Pharmacology-Toxicology, West Virginia University Medical Center, 26506, Morgantown, West Virginia

    Knox Van Dyke & Cynthia J. Van Dyke

  2. Departments of Physiology, West Virginia University Medical Center, 26506, Morgantown, West Virginia

    Vincent Castranova

  3. Departments of Pharmacy, West Virginia University Medical Center, 26506, Morgantown, West Virginia

    Joseph Ma & Kirsten Michaux

  4. Pharmacology and Medicinal Chemistry Division, Abbott Laboratories, Abbott Park, 60064, North Chicago, Illinois

    Karl W. Mollison & George W. Carter

Authors
  1. Knox Van Dyke
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  2. Vincent Castranova
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  3. Cynthia J. Van Dyke
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  4. Joseph Ma
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  5. Kirsten Michaux
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  6. Karl W. Mollison
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  7. George W. Carter
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Van Dyke, K., Castranova, V., Van Dyke, C.J. et al. Granulocyte response to oxidized FMLP. Inflammation 8, 87–99 (1984). https://doi.org/10.1007/BF00918356

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