Skip to main content
SpringerLink
Log in

Expression of the HML-1 Epitope on Human Monocytes is Independent of αE Integrin mRNA

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Interferon gamma (IFNγ)-mediated activation of the myelomonocytic cell line HL-60 and peripheral blood monocytes will induce expression of an epitope for the monoclonal antibody which recognizes human αEβ7 integrin, HML-1. Here, we found that the anti-human αEβ7 monoclonal antibody Ber-ACT8 did not recognize IFNγ-stimulated, HML-1 positive HL-60 cells. Culture of blood monocytes with IFNγ also induced expression of HML-1 but not Ber-ACT8 epitopes. Moreover, migration of monocytes across a monolayer of human airway epithelial cells rapidly induced expression of HML-1, with no detectable Ber-ACT8 staining. Using two different sets of primers specific for the human αE integrin gene, we were unable to detect αE mRNA within HL-60 cells or isolated monocytes by reverse transcriptase polymerase chain reaction methods. We conclude that reactivity of the HML-1 antibody for HL-60 cells and monocytes does not correspond with the expression of the αE integrin subunit, and instead detects a marker for cellular activation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. WRIGHT, S. D., and SILVERSTEIN, S. C. (1982) Tumor-promoting phorbol esters stimulate C3b and C3b' receptor-phagocytosis in cultured human monocytes. J. Exp. Med. 156:1149-1164.

    Google Scholar 

  2. PABST, M. J., HEDEGAARD, H. B., and JOHNSTON, R. B. J. (1982) Cultured human monocytes require exposure to bacterial products to maintain an optimal oxygen radical response. J. Immunol. 128:123-128.

    Google Scholar 

  3. LE, J., PRENSKY, W., YIP, Y. K., CHANG, Z., HOFFMAN, T., STEVENSON, H. C., BALAZS, I., SADLIK, J. R., and VILACEK, J. (1983) Activation of human monocyte cytotoxicity by natural and recombinant immune interferon. J. Immunol. 131:2821-2826.

    Google Scholar 

  4. PRIETO, J., EKLUND, A., and PATARROYO, M. (1994) Regulated expression of integrins and other adhesion molecules during differentiation of monocytes into macrophages. Cell. Immunol. 156:191-211.

    Google Scholar 

  5. TIISALA, S., PAAVENEN, T., and RENKONEN, R. (1995) αEβ7 integrins associated with intraepithelial and mucosal homing, are expressed on macrophages. Eur. J. Immunol. 25:411-417.

    Google Scholar 

  6. CERF-BENSUSSAN, N., JARRY, A., BROUSSE, N., LISOWSKA-GROSPIERRE, B., GUY-GRAND, D., and GRISCELLI, C. (1987) A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur. J. Immunol. 17:1279-1285.

    Google Scholar 

  7. PALLESEN, G., and HAMILTON-DUTOIT, S. (1989) Monoclonal antibody (HML-1) labelling of T-cell lymphomas. Lancet i, 223-224.

    Google Scholar 

  8. SPERLING, M., KAUDEWITZ, P., BRAUN-FELCO, O., and STEIN, H. (1989) Reactivity of T cells in mycosis fungoides exhibiting marked epidermotropism with the monoclonal antibody HML-1 that defines a membrane molecule on human mucosal lymphocytes. Am. J. Pathol. 134:955-960.

    Google Scholar 

  9. MILLER, L. A., and BUTCHER, E. C. (1998) Human airway epithelial monolayers promote selective transmigration of memory T cells: A transepithelial model of lymphocyte migration into the airways. Am. J. Respir. Cell Mol. Biol. 19:892-900.

    Google Scholar 

  10. KRUSCHWITZ, M., FRITZSCHE, G., SCHWARTING, R., MICKLEM, K., MASON, D. Y., FALINI, B., STEIN, H. (1991) Ber-ACT8: New monoclonal antibody to the mucosa lymphocyte antigen. J. Clin. Pathol. 44:636-645.

    Google Scholar 

  11. CEPEK, K. L., PARKER, C. M., MADARA, J. L., and BRENNER, M. B. (1993) Integrin αEβ7 mediates adhesion of T lymphocytes to epithelial cells. J. Immunol. 150:3459-3470.

    Google Scholar 

  12. LUBBERT, M., and KOEFFLER, H. P. (1988) Leukemic cell lines as a paradigm for myeloid differentiation. Cancer Rev. 10:33-62.

    Google Scholar 

  13. GRAZIANI-BOWERING, G. M., GRAHAM, J. M., and FILION, L. G. (1997) A quick, easy and inexpensive method for the isolation of human peripheral blood monocytes. J. Immunol. Methods 207:157-168.

    Google Scholar 

  14. SHAW, S. K., CEPEK, K. L., MURPHY, E. A., RUSSELL, G. J., BRENNER, M. B., and PARKERS, C. M. (1994) Molecular cloning of the human mucosal lymphocyte integrin αE subunit. J. Biol. Chem. 269:6016-6025.

    Google Scholar 

  15. PARKER, C. M., CEPEK, K. L., RUSSELL, G. J., SHAW, S. K., POSNETT, D. N., SCHWARTING, R., and BRENNER, M. B. (1992) A family of beta 7 integrins on human mucosal lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 89:1924-1928.

    Google Scholar 

  16. SPENCER, J., CERF-BENSUSSAN, N., JARRY, A., BROUSSE, N., GUY-GRAND, D., KRAJEWSKI, A. S., and ISAACSON, P. G. (1988) Enteropathy-associated T cell lymphoma (malignant histiocytosis of the intestine) is recognized by a monoclonal antibody (HML-1) that defines a membrane molecule on human mucosal lymphocytes. Am. J. Pathol. 132:1-5.

    Google Scholar 

  17. STEIN, H., DIENEMANN, D., SPERLING, M., ZEITZ, M., and RIECKEN, E. O. (1988) Identification of a T cell lymphoma category derived from intestinal-mucosa-associated T cells. Lancet 2:1053-1054.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Center for Comparative Respiratory Biology and Medicine, Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, UC Davis, Davis, California, 95616

    Lisa A. Miller, Congfen Li & Dallas M. Hyde

Authors
  1. Lisa A. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Congfen Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dallas M. Hyde
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miller, L.A., Li, C. & Hyde, D.M. Expression of the HML-1 Epitope on Human Monocytes is Independent of αE Integrin mRNA. Inflammation 24, 195–205 (2000). https://doi.org/10.1023/A:1007007428255

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1007007428255

Keywords

Search

Navigation