Abstract
The primary structure of the major protein from the Corpuscles of Stannius (CS) of the Australian eel was elucidated from the cDNA sequence and was found to bear close similarity to the N-terminal amino acid sequence of the presumably homologous salmon hormone, teleocalcin (TC). The cDNA sequence predicted a preproprotein of 263 amino acids. Following removal of a 17 amino acid signal peptide, specific monobasic cleavage at an Arg-Phe bond generates the 231 amino acid mature form of the protein. The isolation and sequence determination of the prosequence confirms that the precursor contains a prosegment of 15 residues. Various fragments of the protein have been synthesized chemically and their biological activity assessed. The N-terminal 1–20 fragment of the mature protein inhibits calcium uptake in fingerling trout, the effect being similar, but not equipotent to salmon teleocalcin. Further, infusion of either the N-terminal 1–20 or the 81–94 fragment at 50 μg/h into the renal artery of conscious sheep, caused significant decreases in systemic plasma potassium concentration and in potassium excretion. The 1–20 fragment also gave rise to a small but significant increase in sodium excretion. Infusion of TC at the same rate results in a significant decrease in plasma potassium and phosphate concentration as well as a significant decrease in potassium excretion. Bovine PTH (1–34) at 100 μg/h causes a small decrease in plasma potassium and phosphate and an increase in plasma calcium concentration, and was the only peptide to cause a significant decrease in calcium excretion.
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References cited
Atherton, E., Cameron, L.R. and Sheppard, R.C. 1988. Peptide synthesis. Part 10. Use of pentafluorophenyl esters of fluorenylmethoxycarbonylamino acids in solid phase peptide synthesis. Tetrahedron 44: 843–857.
Benoit, R., Ling, N. and Esch, F. 1982. A new prosomatostatin-derived peptide reveals a pattern for prohormone cleavage at monobasic sites. Science 233: 1126–1129.
Butkus, A., Roche, P.J., Fernley, R.T., Haralambidis, J., Penschow, J.D., Ryan, G.B., Trahair, J.F., Tregear, G.W. and Coghlan, J.P. 1987. Purification and cloning of a corpuscles of Stannius protein fromAnguilla australis. Mol. Cell. Endocrinol. 54: 123–133.
Kreil, G. 1986. The posttranslational processing of the precursors of secreted proteins.In Protein Compartmentalization. pp. 61–70. Edited by A.W. Strauss, I. Boime and G. Kreil. Springer-Verlag, New York.
Krishnamurthy, V.G. and Bern, H.A. 1969. Correlative histological study of the corpuscles of Stannius and the juxtaglomerular cells of teleost fishes. Gen. Comp. Endocrinol. 13: 313–335.
Lafeber, F.P.J.G., Hansen, R.G.J.M., Chdy, Y.M., Flik, G., Herrmann-Erlee, M.P.M., Pang, P.K. and Bonga, S.E.W. 1988. Identification of hypocalcin (teleocalcin) isolated from trout Stannius corpuscles. Gen. Comp. Endocrinol. 69: 19–30.
Lopez, E., Tisserand-Jochem, E.-M., Eyquem, A., Milet, C., Hillyard, C., Lallier, F., Vidal, B. and MacIntyre, I. 1984. Immunocytochemical detection in eel corpuscles of Stannius of a mammalian parathyroid-like hormone. Gen. Comp. Endocrinol. 53: 28–36.
Ma, S.W.Y. and Copp, D.H. 1978. Purification, properties and action of a glycopeptide from the corpuscles of Stannius which effect calcium metabolism in the teleost.In Comparative Endocrinology. pp. 283–386. Edited by P.H. Gaillard and H.H. Boer. Elsevier/North-Holland Biomedical Press, Amsterdam.
Merrifield, R.G. 1985. Solid phase synthesis. Angew. Chem. Int. Ed. Engl. 24: 799–892.
Millet, C., Hillyard, C.J., Martelly, E., Grigis, G., MacIntyre, I. and Lopez, E. 1980. Similitudes structureles entre l'hormone hypocalcémiante des corpuscles de Stannius (PCS) de l'anguille (Anguilla anguilla L.) et l'hormone parathyroidienne mammalienne. C.R. Acad. Sci. Ser. D 291: 977–980.
Milet, C., Hillyard, C.J., Martelly, E., Chartier, M.M., Grigis, S., McIntyre, I. and Lopez, E. 1982. A parathyroid-like hormone from eel corpuscles of Stannius which exhibits hypocalcemic actionIn Comparative Endocrinology of Calcium Regulation pp. 181–185. Edited by C. Oguro and P.K.T. Pang. Japanese Scientific Society Press, Tokyo.
O'Farrell, P.H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007–4021.
Von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14: 4683–4690.
Wagner, G.F., Hampong, M., Park, C.M. and Copp, D.H. 1986. Purification, characterization, and bioassay of teleocalcin, a glycoprotein from salmon corpuscles of Stannius. Gen. Comp. Endocrinol. 63: 481–491.
Yates, N.A., McDougall, J.G., Coghlan, J.P., Coker, A.R., Gibson, A.P. and Scoggins, B.A. 1988. Renal effects of atrial natriuretic factor (99–126) in conscious Na replete sheep. Clin. Exp. Pharmacol. Physiol. 15: (In press).
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Butkus, A., Yates, N.A., Copp, D.H. et al. Processing and bioactivity of the corpuscles of Stannius protein of the Australian eel. Fish Physiol Biochem 7, 359–365 (1989). https://doi.org/10.1007/BF00004729
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DOI: https://doi.org/10.1007/BF00004729